CNRS – offre d’emploi IE

Bacterial conjugation is a major horizontal gene transfer mechanism through which DNA is transferred from a donor to a recipient bacterium. Conjugation has important life science implications including bacterial genome evolution, dissemination of new genetic traits (including drug resistance) and bioengineering applications. The fundamental steps of conjugational DNA transfer are highly conserved: (1) establishment of donor-recipient mating pair, (2) extrusion of a single-strand (ss) DNA in the donor cell, (3) transfer of the linear ssDNA from the donor to the recipient cell, (4) reconstitution of a circular double-strand (ds) plasmid molecule in the recipient cell. Subsequently, (5) the newly acquired plasmid is faithfully maintained over generations, while the expression of plasmid genes phenotypically converts the recipient cell into a new donor cell. Although it has been studied for many years, we still lack a comprehensive understanding of the conjugation mechanisms. Particularly, our recent results suggested a current knowledge gap at (4), where an intriguing interplay between dsDNA conversion/DNA replication and transcription/translation of plasmid factors must take place.

We are looking for an engineer with IE level, to participate our ANR project which aims to provide an integrated view of plasmid establishment in the recipient cell following its conjugal transfer. The recruited person will carry out experiments (i) to characterize the dynamics of ssDNA entry, ss to ds DNA conversion, and transcription and translation of genes in different plasmid loci, and (ii) to identify plasmid and host factors involved in the process of conjugation.
 
We expect the contract will start from 1st of September (+/- 1 month) and for 24-months.
 
Please do not hesitate to contact Yoshiharu.Yamaichi@i2bc.paris-saclay.fr for any inquiries.

Veuillez postuler directement en vous adressant au contact indiqué dans l’offre ci-avant. L’ESTBA ne traitera aucune candidature. Merci.